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Antibodies
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anti-Chromogranin A (CHGA) antibody
Chromogranin A (CHGA) antibody
| Antigen |
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| Synonyms |
CGA, MGC105435, fj05f09, wu:fj05f09, zgc:101749, si:dkey-177p2.2, CHGA |
| Clonality |
Monoclonal (SP12) |
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Host
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Reactivity
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Application
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Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN125278 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
207.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Chromogranin A
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Immunogen
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Recombinant protein encoding human Chromogranin A.
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Format
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Supernatant
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Isotype
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IgG (Matching secondary antibodies)
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Clone
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SP12
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Specificity
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This antibody recognizes Chromogranin A. Chromogranin A (a protein of 439 amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.This antibody crossreacts with Human.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Pancreas. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cancer, Neurotransmitters
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Chromogranin A (CHGA)", type "Antibodies"
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Hosts
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Mouse (22), Rabbit (21), Goat (5), Sheep (2), Chicken (1)
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Reactivities
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Human (46), Rat (Rattus) (9), Monkey (8), Pig (Porcine) (7), Mouse (Murine) (6), Rabbit (6)
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Applications
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (25), Western Blotting (WB) (19), Immunohistochemistry (IHC) (14), ELISA (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (9), Enzyme Immunoassay (EIA) (7), Immunofluorescence (IF) (4), Flow Cytometry (FACS) (2), Cell-ELISA (cELISA) (1), ELISA (Detection) (1)
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