Placental Alkaline Phosphatase (PLAP) antibody

Antigen: Placental Alkaline Phosphatase (PLAP)

Synonyms: DOA1, PLAP, PLA2P, FLJ11281, FLJ12699, cb623, zgc:56568, zgc:66193, wu:fc45d08, wu:fi33e06, PLAA

Clonality: Monoclonal (SP15)

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125280

Additional Information

Immunogen: Recombinant protein encoding human Placental Alkaline Phosphatase (PLAP).

Format: Supernatant

Isotype: IgG  (Matching secondary antibodies)

Clone: SP15

Specificity: This antibody reacts with a membrane-bound isoenzyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. This antibody is highly specific to PLAP and shows no cross-reaction with other isoenzymes of alkaline phosphatases. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).This antibody crossreacts with Human, Mouse and Rat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Placenta. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Enzymes

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union