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Epidermal Growth Factor Receptor (EGFR) antibody

Antigen

Epidermal Growth Factor Receptor (EGFR)

Synonyms
ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33,  ... show more
Clonality Monoclonal (SP9)
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human

Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125283
Quantity 500ul  (100ug/ml approximately)  (Variants)
Price 283.00 $   Plus shipping costs $35.00
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Additional Information

Immunogen A synthetic peptide from C- terminus of human EGFR protein.
Format Supernatant
Isotype IgG  (Matching secondary antibodies)
Clone SP9
Specificity This antibody recognizes EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, 2, 3, -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-alpha (TGF-alpha), Heparin-binding EGF (HB- EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head, neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.This antibody crossreacts with Human.
Synonyms ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Application Details

Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Placenta, Lung. Cellular localization: Cytoplasmic, cell membrane Other Applications: Immunoprecipitation and Western blotting (1:100-500).
Concentration 100ug/ml approximately
Buffer This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives

Alternatives for antigen "Epidermal Growth Factor Receptor (EGFR)", type "Antibodies"
Hosts Rabbit (391), Mouse (166), Rat (8), Sheep (7), Goat (2)
Reactivities Human (555), Mouse (Murine) (263), Rat (Rattus) (238), Dog (Canine) (33), Rabbit (18), Pig (Porcine) (17), Cow (Bovine) (6), Chicken (5), Horse (Equine) (2), Sheep (Ovine) (2)
Applications Western Blotting (WB) (401), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (131), Immunoprecipitation (IP) (120), Enzyme Immunoassay (EIA) (103), Immunohistochemistry (IHC) (103), Immunofluorescence (IF) (95), ELISA (92), Flow Cytometry (FACS) (90), Immunohistochemistry (Frozen Sections) (IHC (fro)) (40), Blocking Antibody (Inhibition) (16), Dot Blot (Dot) (14), Immunocytochemistry (ICC) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Functional Studies (Func) (3), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Neutralization (Neut) (2), Blocking Antibody (Inhibition) (1)
Conjugates Biotin (35), FITC (5), Alexa Fluor 488 (3), PE (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
Epitopes pTyr1197 (17), C-Term (16), pTyr869 (13), pTyr1092 (12), pTyr1110 (11), pTyr1172 (11), pThr678 (10), pSer1070 (9), pThr693 (9), N-Term (5), pTyr1016 (3), pTyr1069 (3), AA 1-203 (2), AA 1140-1160 (2), AA 960-980 (2), Extracell. non Ligand binding Site (2), S695 (2), Y1125 (2), Y869 (2), Y998 (2), pSer1071 (2), pTyr1173 (2), AA 1189-1199 (1), AA 985-996 (1), Ab-1016 (1), Ab-1026 (1), Ab-1069 (1), Ab-1071 (1), Amino Acids 1165-1186 (1), External Domain (1), Extracellular non-ligand binding site (1), S1026 (1), S1070 (1), S1071 (1), S1166 (1), S1190 (1), S991 (1), T678 (1), TYR1173 (1), Y1016 (1), Y1069 (1), Y1092 (1), Y1110 (1), Y1172 (1), Y978 (1), extracellular ligand binding site (1), internal (1), pS1070 (1), pS768 (1), pSer1026 (1), pSer1166 (1), pSer1190 (1), pSer695 (1), pSer768 (1), pTyr1125 (1), pTyr992 (1), pTyr998 (1), pY1016 (1), pY1092 (1), pY1172 (1), prylated At Threonine 654 (1), prylated At Tyrosine 1045 (1), prylated At Tyrosine 1173 (1), prylated At Tyrosine 845 (1), prylated at Serine 1047 (1)