COX-2 antibody
| Antigen |
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| Clonality |
Monoclonal (SP21) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN125290 |
| Quantity |
6ml (1 to 4 ug/ml) (Variants) |
| Price |
272.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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Synthetic peptide corresponding to C-terminus of COX2.
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Format
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Supernatant (prediluted)
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Isotype
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IgG (Matching secondary antibodies)
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Clone
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SP21
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Specificity
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COX2 (Cyclooxygenase-2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate-early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged. This antibody crossreacts with Human, Mouse and rat.
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Application Notes
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Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Lung carcinoma, Colon carcinoma. Cellular localization: Cytoplasmic, membrane
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Concentration
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1 to 4 ug/ml
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Buffer
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This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "COX-2", type "Antibodies"
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Hosts
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Rabbit (15), Mouse (2)
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Reactivities
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Human (12), Rat (Rattus) (6), Mouse (Murine) (1), Sheep (Ovine) (1)
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Applications
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Western Blotting (WB) (15), Immunohistochemistry (IHC) (11), Immunofluorescence (IF) (3), Immunoprecipitation (IP) (2), ELISA (1), Immunocytochemistry (ICC) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)
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