Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) antibody

Antigen: Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2)

Synonyms: CD23, FCE2, CD23A, IGEBF, CLEC4J, Fce2, Fcer2, Ly-42

Clonality: Monoclonal (SP23)

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125297

Additional Information

Alternative name: CD23

Immunogen: Recombinant protein encoding human CD23 48-248aa.

Format: Supernatant (prediluted)

Isotype: IgG

Clone: SP23

Specificity: This antibody recognizes CD23. CD23 is a 45kDa glycoprotein, which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma.This antibody crossreacts with Human.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union