Synaptophysin (SYP) antibody
| Antigen |
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| Synonyms |
Syn, p38, AI848995, A230093K24Rik, Syp1, syp-A, xsypi, MGC86277, syp-B, xsypii, MGC86267 |
| Clonality |
Monoclonal (SP11) |
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Host
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Reactivity
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Application
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Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (45), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (36), Immunohistochemistry (IHC) (22), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), ELISA (8), Enzyme Immunoassay (EIA) (8), Flow Cytometry (FACS) (5), Immunocytochemistry (ICC) (3), Immunofluorescence (IF) (3), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1)
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| Catalog no. |
ABIN125302 |
| Quantity |
6ml (1 to 4 ug/ml) (Variants) |
| Price |
168.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Synaptophysin
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Immunogen
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A synthetic peptide of human Synaptophysin.
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Format
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Supernatant (prediluted)
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Isotype
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IgG (Matching secondary antibodies)
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Clone
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SP11
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Specificity
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This antibody recognizes a protein of 38kDa, identified as synaptophysin. It labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneths cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. In combination with anti-chromogranin A and anti-NSE, Ab to synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.This antibody crossreacts with Human.
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Application Notes
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Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Pancreas. Cellular localization: Cytoplasmic
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Concentration
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1 to 4 ug/ml
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Buffer
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This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Synaptophysin (SYP)", type "Antibodies"
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Hosts
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Rabbit (43), Mouse (22), Sheep (4)
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Reactivities
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Human (57), Rat (Rattus) (26), Mouse (Murine) (12), Cow (Bovine) (5), Hamster (2), Pig (Porcine) (2), Chicken (1), Monkey (1)
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Applications
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Western Blotting (WB) (45), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (36), Immunohistochemistry (IHC) (22), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), ELISA (8), Enzyme Immunoassay (EIA) (8), Flow Cytometry (FACS) (5), Immunocytochemistry (ICC) (3), Immunofluorescence (IF) (3), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1)
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Conjugates
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Biotin (1), FITC (1)
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Epitopes
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C-Term (7), N-Term (5), internal (4), AA 245-258 (1), Y81 (1), pTyr81 (1), ppY81 (1)
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