Vimentin (VIM) antibody
| Antigen |
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| Synonyms |
FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime |
| Clonality |
Monoclonal (SP20) |
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Host
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Reactivity
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Human (144), Rat (Rattus) (66), Mouse (Murine) (60), Chicken (31), Cow (Bovine) (26), Pig (Porcine) (23), Dog (Canine) (9), Mammalian (8), Monkey (6), Goat (5), Hamster (5), Amphibian (4), Potoroo (2), Fish (1), Primate (1), Rabbit (1), Xenopus laevis (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (90), Immunofluorescence (IF) (68), Immunohistochemistry (IHC) (55), Flow Cytometry (FACS) (54), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (52), ELISA (39), Immunohistochemistry (Frozen Sections) (IHC (fro)) (26), Immunocytochemistry (ICC) (13), Immunoprecipitation (IP) (11), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Transfected cell culture (TCC) (2), Dot Blot (Dot) (1)
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| Catalog no. |
ABIN125303 |
| Quantity |
6ml (1 to 4 ug/ml) (Variants) |
| Price |
128.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Vimentin
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Immunogen
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Recombinant human vimentin protein.
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Format
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Supernatant (prediluted)
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Isotype
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IgG (Matching secondary antibodies)
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Clone
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SP20
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Specificity
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This antibody recognizes vimentin. Vimentin is the main intermediate filament protein in mesenchymal cells and is therefore of value in the differential diagnosis of undifferentiated neoplasms. This antibody crossreacts with Human.
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Application Notes
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Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. No pretreatment is required for formalin fixed paraffin tissue sections. Positive Control: Sarcomas. Cellular localization: Cytoplasmic
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Concentration
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1 to 4 ug/ml
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Buffer
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This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cell/Tissue Markers, Cytoskeleton
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Vimentin (VIM)", type "Antibodies"
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Hosts
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Rabbit (88), Mouse (64), Chicken (8), Sheep (3), Guinea Pig (2), Goat (1)
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Reactivities
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Human (144), Rat (Rattus) (66), Mouse (Murine) (60), Chicken (31), Cow (Bovine) (26), Pig (Porcine) (23), Dog (Canine) (9), Mammalian (8), Monkey (6), Goat (5), Hamster (5), Amphibian (4), Potoroo (2), Fish (1), Primate (1), Rabbit (1), Xenopus laevis (1)
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Applications
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Western Blotting (WB) (90), Immunofluorescence (IF) (68), Immunohistochemistry (IHC) (55), Flow Cytometry (FACS) (54), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (52), ELISA (39), Immunohistochemistry (Frozen Sections) (IHC (fro)) (26), Immunocytochemistry (ICC) (13), Immunoprecipitation (IP) (11), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Transfected cell culture (TCC) (2), Dot Blot (Dot) (1)
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Conjugates
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FITC (9), Biotin (8), Alexa Fluor 488 (4), PE (4), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), DY547 (1), Dyomics 547 (1)
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Epitopes
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pSer82 (20), C-Term (2), Center (2), S82 (2), Ab-56 (1), Ab-83 (1), pSer56 (1), pSer83 (1)
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