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Epidermal Growth Factor Receptor (EGFR) antibody

Antigen

Epidermal Growth Factor Receptor (EGFR)
Synonyms
ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33,  ... show more
Clonality Monoclonal (SP9)
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125304
Quantity 6ml  (1 to 4 ug/ml)  (Variants)
Price 162.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Immunogen A synthetic peptide from C-terminus of human EGFR protein.
Format Supernatant (prediluted)
Isotype IgG  (Matching secondary antibodies)
Clone SP9
Specificity This antibody recognizes EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, 02, 03, -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-alpha (TGF-alpha), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head, neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.This antibody crossreacts with Human.
Synonyms ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. No pretreatment is required for formalin fixed paraffin tissue sections. Positive Control: Placenta, Lung. Cellular localization: Cytoplasmic, cell membrane
Concentration 1 to 4 ug/ml
Buffer This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Epidermal Growth Factor Receptor (EGFR)", type "Antibodies"
Hosts Rabbit (391), Mouse (166), Rat (8), Sheep (7), Goat (2)
Reactivities Human (555), Mouse (Murine) (263), Rat (Rattus) (238), Dog (Canine) (33), Rabbit (18), Pig (Porcine) (17), Cow (Bovine) (6), Chicken (5), Horse (Equine) (2), Sheep (Ovine) (2)
Applications Western Blotting (WB) (401), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (131), Immunoprecipitation (IP) (120), Enzyme Immunoassay (EIA) (103), Immunohistochemistry (IHC) (103), Immunofluorescence (IF) (95), ELISA (92), Flow Cytometry (FACS) (90), Immunohistochemistry (Frozen Sections) (IHC (fro)) (40), Blocking Antibody (Inhibition) (16), Dot Blot (Dot) (14), Immunocytochemistry (ICC) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Functional Studies (Func) (3), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Neutralization (Neut) (2), Blocking Antibody (Inhibition) (1)
Conjugates Biotin (35), FITC (5), Alexa Fluor 488 (3), PE (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
Epitopes pTyr1197 (17), C-Term (16), pTyr869 (13), pTyr1092 (12), pTyr1110 (11), pTyr1172 (11), pThr678 (10), pSer1070 (9), pThr693 (9), N-Term (5), pTyr1016 (3), pTyr1069 (3), AA 1-203 (2), AA 1140-1160 (2), AA 960-980 (2), Extracell. non Ligand binding Site (2), S695 (2), Y1125 (2), Y869 (2), Y998 (2), pSer1071 (2), pTyr1173 (2), AA 1189-1199 (1), AA 985-996 (1), Ab-1016 (1), Ab-1026 (1), Ab-1069 (1), Ab-1071 (1), Amino Acids 1165-1186 (1), External Domain (1), Extracellular non-ligand binding site (1), S1026 (1), S1070 (1), S1071 (1), S1166 (1), S1190 (1), S991 (1), T678 (1), TYR1173 (1), Y1016 (1), Y1069 (1), Y1092 (1), Y1110 (1), Y1172 (1), Y978 (1), extracellular ligand binding site (1), internal (1), pS1070 (1), pS768 (1), pSer1026 (1), pSer1166 (1), pSer1190 (1), pSer695 (1), pSer768 (1), pTyr1125 (1), pTyr992 (1), pTyr998 (1), pY1016 (1), pY1092 (1), pY1172 (1), prylated At Threonine 654 (1), prylated At Tyrosine 1045 (1), prylated At Tyrosine 1173 (1), prylated At Tyrosine 845 (1), prylated at Serine 1047 (1)