Prostate Apoptosis Response-4 (Par-4) antibody

Antigen: Prostate Apoptosis Response-4 (Par-4)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125320

Additional Information

Immunogen: A synthetic peptide derived from the C-terminal of human Par-4 protein.

Format: Ig-fraction

Specificity: Prostate apoptosis response-4 (Par-4) is a 38kD protein originally identified as the product of a gene that is upregulated in prostate tumor cells undergoing apoptosis. It is a leucine zipper and death domain-containing protein whose levels increase in neurons undergoing apoptosis as a result of trophic factor withdrawal or exposure to oxidative and metabolic insults. Par-4 levels are reported to be increased in lumbar spinal cord specimens from patients with amyotrophic lateral sclerosis (ALS)1 further suggesting a role in neuronal degeneration. This antibody cross reacts with mouse and rat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Myocardium. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against Par-4 protein containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human heart stained with Par-4 antibody