Thyroid Hormone Receptor, Alpha1/Alpha2 (THRA1/2) antibody

Antigen: Thyroid Hormone Receptor, Alpha1/Alpha2 (THRA1/2)

Synonyms: LOC397942, thra1, thra-A, nr1a1-A, THRB, NR1A2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125322

Additional Information

Alternative name: Thyroid Hormone Receptor-alpha1/alpha2 (THR alpha1/alpha2)

Immunogen: A synthetic peptide derived from aa 13-32 of human TR-alpha protein.

Format: Ig-fraction

Specificity: Thyroid hormone receptors (TRs) are ligand dependent members of the steroid/retinoic acid superfamily of transcription factors. The two genes encoding TRs identified to date, TR alpha and TR Beta, have been mapped to human chromosomes 17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) with half site binding motifs in the orientation of palindromes, direct repeats, or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3-triiodo-L-thyronine (T-3). This antibody cross reacts with mouse, rat and pig.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against TRs alpha protein containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Hormones, Receptors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human Colon carcinoma stained with THR a1/a2 using ABC and AEC chromogen.