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Claudin 5 (CLDN5) antibody
| Antigen | Claudin 5 (CLDN5) |
| Synonyms | AWAL, BEC1, TMVCF, CPETRL1, awal, bec1, tmvcf, cpetrl1, MGC89185, claudin-5, cld5, MBEC1, Tmvcf, AI854493, MGC102088, MGC95240, CLDN5, cldn5, MGC138054 |
| Clonality | Polyclonal |
| Host |
 Alternatives Rabbit |
| Reactivity |
Alternatives Human |
| Application |
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|
| Catalog no. | ABIN125330 |
| Quantity | 1ml (100ug/ml approximately) (Variants) |
| Price | 406.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | Claudin 5 |
| Immunogen | A synthetic peptide corresponding to C-terminal of human Claudin 5. |
| Format | Ig-fraction |
| Specificity | Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact, made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition. This antibody cross reacts with human and mouse. |
Application Details
| Application Notes | Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Lung. Cellular localization: Cell membrane, cytoplasmic |
| Concentration | 100ug/ml approximately |
| Buffer | Purified immunoglobulin fraction of rabbit antiserum against Claudin 5 protein containing sodium azide as preservative. |
| Storage | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Research Area | Cytoskeleton |
| Restrictions | For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union |
Alternatives
Alternatives for antigen "Claudin 5 (CLDN5)", type "Antibodies"
| Hosts | Rabbit (54), Mouse (1) |
| Reactivities | Human (52), Mouse (Murine) (48), Rat (Rattus) (41), Dog (Canine) (33), Pig (Porcine) (31), Rabbit (30), Cow (Bovine) (18), Horse (Equine) (15) |
| Applications | Immunofluorescence (IF) (39), Flow Cytometry (FACS) (37), Western Blotting (WB) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), ELISA (8), Immunohistochemistry (IHC) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoprecipitation (IP) (4), Enzyme Immunoassay (EIA) (2), Immunoelectron Microscopy (IEM) (2) |
| Conjugates | Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2) |
| Epitopes | C-Term (6), pTyr217 (1), transcript variant 2 (1) |
