Cytokeratin 1 (KRT1) antibody
| Antigen |
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| Synonyms |
K1, CK1, EHK, EHK1, EPPK, KRT1A, NEPPK |
| Clonality |
Monoclonal (KL1) |
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Host
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Reactivity
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Human (30), Rat (Rattus) (6), Chicken (4), Cow (Bovine) (4), Mouse (Murine) (3), Rabbit (3), Frog (2), Mammalian (2), Snake (2), Dog (Canine) (1), Hamster (1), Horse (Equine) (1), Monkey (1), Pig (Porcine) (1), Salmonella typhi (S. typhi) (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN125333 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
197.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Cytokeratin
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Immunogen
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Human keratin isolated from epidermal stratum corneum.
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Format
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Supernatant
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Isotype
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IgG1 kappa (Matching secondary antibodies)
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Clone
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KL1
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Specificity
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This antibody is specific to 56 kD cytokeratin. Immunoblotting analysis revealed that this antibody reacts with cytokeratin 1, 2, 5, 6, 7, 8, 11, 14, 16, 17 and 18. This antibody stains with neoplastic cells of epithelial origin. Non-epithelial cells and skin basal layers cells do not stain with this antibody.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment prior to immunostaining of formalin fixed paraffin embedded tissue sections may enhance staining intensity. Positive Control: Skin. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Extracellular Matrix
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Cytokeratin 1 (KRT1)", type "Antibodies"
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Hosts
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Mouse (32), Guinea Pig (2), Rabbit (2)
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Reactivities
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Human (30), Rat (Rattus) (6), Chicken (4), Cow (Bovine) (4), Mouse (Murine) (3), Rabbit (3), Frog (2), Mammalian (2), Snake (2), Dog (Canine) (1), Hamster (1), Horse (Equine) (1), Monkey (1), Pig (Porcine) (1), Salmonella typhi (S. typhi) (1)
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Applications
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (11), Western Blotting (WB) (10), Immunohistochemistry (IHC) (6), Flow Cytometry (FACS) (3), Immunofluorescence (IF) (3), Enzyme Immunoassay (EIA) (2), ELISA (1), Immunocytochemistry (ICC) (1)
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Conjugates
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APC (1), PE (1)
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