»
Antibodies
»
anti-Glial Fibrillary Acidic Protein (GFAP) antibody
Glial Fibrillary Acidic Protein (GFAP) antibody
| Antigen |
|
| Synonyms |
GFAP, DKFZp459C0729, MGC139638, FLJ45472, AI836096, cb345, gfapl, MGC110485, wu:fb34h11, wu:fk42c12, zgc:110485, etID36982.3, xx:af506734 |
| Clonality |
Monoclonal (6F2) |
|
Host
|
|
|
Reactivity
|
Human (164), Rat (Rattus) (69), Pig (Porcine) (66), Mouse (Murine) (51), Cow (Bovine) (46), Sheep (Ovine) (21), Rabbit (19), Dog (Canine) (18), Chicken (13), Guinea Pig (12), Mammalian (11), Cat (Feline) (8), Goat (3), Bird (Avian) (2), Caprinae (2), Hamster (1), Horse (Equine) (1), Monkey (1), Primate (1)
|
|
Application
|
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (152), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (106), Immunofluorescence (IF) (99), Immunohistochemistry (Frozen Sections) (IHC (fro)) (62), Immunohistochemistry (IHC) (46), Immunoprecipitation (IP) (31), Flow Cytometry (FACS) (23), Immunocytochemistry (ICC) (21), ELISA (18), Enzyme Immunoassay (EIA) (15), ELISA (Detection) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Capture) (1), Immunoelectron Microscopy (IEM) (1)
|
| Catalog no. |
ABIN125339 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
206.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Immunogen
|
Glial fibrillary acidic protein from human brain.
|
|
Format
|
Supernatant
|
|
Isotype
|
IgG1 kappa (Matching secondary antibodies)
|
|
Clone
|
6F2
|
|
Specificity
|
This antibody is specific to a 52 kD intermediate filament protein in brain and spinal cord. It stains astrocytes and CNS ependymal cells but does not stain oligodendrocytes or neurons. This antibody does not cross react with any other intermediate filament proteins. However, it cross reacts with GFAP from mouse and rabbit.
|
|
Application Notes
|
Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Brain. Cellular localization: Cytoplasm
|
|
Concentration
|
100ug/ml approximately
|
|
Buffer
|
This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
|
|
Storage
|
Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
|
|
Research Area
|
Cell/Tissue Markers, Cytoskeleton, Neurology
|
|
Restrictions
|
For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
|
Alternatives for antigen "Glial Fibrillary Acidic Protein (GFAP)", type "Antibodies"
|
Hosts
|
Mouse (150), Rabbit (82), Chicken (12), Goat (4), Guinea Pig (2)
|
|
Reactivities
|
Human (164), Rat (Rattus) (69), Pig (Porcine) (66), Mouse (Murine) (51), Cow (Bovine) (46), Sheep (Ovine) (21), Rabbit (19), Dog (Canine) (18), Chicken (13), Guinea Pig (12), Mammalian (11), Cat (Feline) (8), Goat (3), Bird (Avian) (2), Caprinae (2), Hamster (1), Horse (Equine) (1), Monkey (1), Primate (1)
|
|
Applications
|
Western Blotting (WB) (152), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (106), Immunofluorescence (IF) (99), Immunohistochemistry (Frozen Sections) (IHC (fro)) (62), Immunohistochemistry (IHC) (46), Immunoprecipitation (IP) (31), Flow Cytometry (FACS) (23), Immunocytochemistry (ICC) (21), ELISA (18), Enzyme Immunoassay (EIA) (15), ELISA (Detection) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Capture) (1), Immunoelectron Microscopy (IEM) (1)
|
|
Conjugates
|
Biotin (6), Cy3 (3), HRP (3), Alexa Fluor 488 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitopes
|
C-Term (1), internal (1), pSer8 (1)
|