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Glial Fibrillary Acidic Protein (GFAP) antibody

Antigen

Glial Fibrillary Acidic Protein (GFAP)

Synonyms GFAP, DKFZp459C0729, MGC139638, FLJ45472, AI836096, cb345, gfapl, MGC110485, wu:fb34h11, wu:fk42c12, zgc:110485, etID36982.3, xx:af506734
Clonality Monoclonal (6F2)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human

Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125339
Quantity 500ul  (100ug/ml approximately)  (Variants)
Price 206.00 $   Plus shipping costs $35.00
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Additional Information

Immunogen Glial fibrillary acidic protein from human brain.
Format Supernatant
Isotype IgG1 kappa  (Matching secondary antibodies)
Clone 6F2
Specificity This antibody is specific to a 52 kD intermediate filament protein in brain and spinal cord. It stains astrocytes and CNS ependymal cells but does not stain oligodendrocytes or neurons. This antibody does not cross react with any other intermediate filament proteins. However, it cross reacts with GFAP from mouse and rabbit.

Application Details

Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Brain. Cellular localization: Cytoplasm
Concentration 100ug/ml approximately
Buffer This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area Cell/Tissue Markers, Cytoskeleton, Neurology
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives

Alternatives for antigen "Glial Fibrillary Acidic Protein (GFAP)", type "Antibodies"
Hosts Mouse (150), Rabbit (82), Chicken (12), Goat (4), Guinea Pig (2)
Reactivities Human (164), Rat (Rattus) (69), Pig (Porcine) (66), Mouse (Murine) (51), Cow (Bovine) (46), Sheep (Ovine) (21), Rabbit (19), Dog (Canine) (18), Chicken (13), Guinea Pig (12), Mammalian (11), Cat (Feline) (8), Goat (3), Bird (Avian) (2), Caprinae (2), Hamster (1), Horse (Equine) (1), Monkey (1), Primate (1)
Applications Western Blotting (WB) (152), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (106), Immunofluorescence (IF) (99), Immunohistochemistry (Frozen Sections) (IHC (fro)) (62), Immunohistochemistry (IHC) (46), Immunoprecipitation (IP) (31), Flow Cytometry (FACS) (23), Immunocytochemistry (ICC) (21), ELISA (18), Enzyme Immunoassay (EIA) (15), ELISA (Detection) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Capture) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (6), Cy3 (3), HRP (3), Alexa Fluor 488 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes C-Term (1), internal (1), pSer8 (1)