Mouse anti-Human Immunoglobulin lambda (IgL) Antibody

Antigen: Immunoglobulin lambda (IgL)

Clonality: Monoclonal (HP6054)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125341

Additional Information

Alternative name: Immunoglobulin Lambda

Immunogen: BALB/C mice were injected with purified human IgG myeloma proteins covalently coupled to polyaminostyren microbeads.

Format: Supernatant

Isotype: IgG2a  (Matching secondary antibodies)

Clone: HP6054

Specificity: This antibody is specific to human lambda light chain. It reacts with free and bound heavy chain isotypes. Lambda light chains are found on the surface of B cells in lymphoid tissues. This antibody is useful for the identification of immunoglobulins in leukemias, plasmacytomas and some non-Hodgkin

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union