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Tumor Protein P53 (TP53) antibody

Antigen

Tumor Protein P53 (TP53)
Synonyms p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53
Clonality Monoclonal (Bp53-12)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125350
Quantity 500ul  (100ug/ml approximately)  (Variants)
Price 239.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name p53
Immunogen Recombinant wild type p53 protein.
Format Supernatant
Isotype IgG2a  (Matching secondary antibodies)
Clone Bp53-12
Specificity This antibody is specific to human p53 protein, both wild and mutant forms under denaturing and non-denaturing conditions. It maps the n-terminus region of the p53 molecule in the region of amino acids 20 and 25.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear
Concentration 100ug/ml approximately
Buffer This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area Transcription Factors
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Tumor Protein P53 (TP53)", type "Antibodies"
Hosts Rabbit (255), Mouse (79), Sheep (3)
Reactivities Human (328), Mouse (Murine) (68), Rat (Rattus) (62), Monkey (5), Cat (Feline) (3), Cow (Bovine) (3), Horse (Equine) (3), Dog (Canine) (2), Hamster (2), Primate (2), Primates (2), Sheep (Ovine) (2), Rabbit (1), Xenopus laevis (1)
Applications Western Blotting (WB) (279), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (85), Immunohistochemistry (IHC) (81), ELISA (68), Immunofluorescence (IF) (61), Immunoprecipitation (IP) (41), Enzyme Immunoassay (EIA) (40), Flow Cytometry (FACS) (27), Immunohistochemistry (Frozen Sections) (IHC (fro)) (26), Immunocytochemistry (ICC) (11), Dot Blot (Dot) (8), Transfected cell culture (TCC) (3), Radioimmunoassay (RIA) (2), Chromatin Immunoprecipitation (ChiP) (1)
Conjugates FITC (7), Biotin (2), HRP (1), PE (1)
Epitopes pSer37 (11), pSer315 (10), pSer33 (9), pSer46 (9), pThr18 (9), pSer392 (8), pSer6 (8), pSer15 (7), AA 371-380 (5), pSer20 (5), pSer32/Ser36 (5), AA 20-25 (4), pSer1232 (4), AA 18-30 (3), C-Term (3), S315 (2), S37 (2), S46 (2), T55 (2), pSer9 (2), AA 16-30 (1), AA 378-393 (1), AA 46-53 (1), AA 46-55 (1), Acetyl-Lys317 (1), Acetyl-Lys379 (1), S15 (1), S376 (1), S378 (1), S9 (1), T18 (1), amino acids 211-220 (1), pS20 (1), pS33 (1), pSer376 (1), pSer378 (1), pThr55 (1), phospho (1)