Insulin (INS) antibody
| Antigen |
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| Synonyms |
ILPR, IRDN, INS, MGC159719, ins1, xins, ins1-a, Insulin |
| Clonality |
Monoclonal (K36ac10) |
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Host
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Reactivity
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Human (159), Pig (Porcine) (92), Rat (Rattus) (59), Mouse (Murine) (45), Cow (Bovine) (41), Rabbit (29), Horse (Equine) (22), Dog (Canine) (9), Chicken (3), Guinea Pig (3), Sheep (Ovine) (3), Cat (Feline) (2)
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Application
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Immunofluorescence (IF) (77), Flow Cytometry (FACS) (71), ELISA (51), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (35), Enzyme Immunoassay (EIA) (34), Radioimmunoassay (RIA) (25), Western Blotting (WB) (25), Immunohistochemistry (Frozen Sections) (IHC (fro)) (21), Immunohistochemistry (IHC) (16), Immunoprecipitation (IP) (13), ELISA (Capture) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Functional Studies (Func) (4), Immunocytochemistry (ICC) (4), Immunoelectron Microscopy (IEM) (3)
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| Catalog no. |
ABIN125360 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
166.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Insulin
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Immunogen
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Human insulin.
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Format
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Supernatant
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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K36ac10
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Specificity
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This antibody recognizes purified insulin from the pancreas of human, bovine, horse, sheep as well as proinsulin from human. Cross reaction has been observed with insulin containing cells in fixed sections of pancreas from human, porcine, dog, rabbit, bovine, sheep, rat, guinea pig and cat.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Pancreas. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as diluted ascites containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Diabetes, Hormones
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Insulin (INS)", type "Antibodies"
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Hosts
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Rabbit (89), Mouse (82), Guinea Pig (8), Sheep (4), Chicken (1)
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Reactivities
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Human (159), Pig (Porcine) (92), Rat (Rattus) (59), Mouse (Murine) (45), Cow (Bovine) (41), Rabbit (29), Horse (Equine) (22), Dog (Canine) (9), Chicken (3), Guinea Pig (3), Sheep (Ovine) (3), Cat (Feline) (2)
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Applications
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Immunofluorescence (IF) (77), Flow Cytometry (FACS) (71), ELISA (51), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (35), Enzyme Immunoassay (EIA) (34), Radioimmunoassay (RIA) (25), Western Blotting (WB) (25), Immunohistochemistry (Frozen Sections) (IHC (fro)) (21), Immunohistochemistry (IHC) (16), Immunoprecipitation (IP) (13), ELISA (Capture) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Functional Studies (Func) (4), Immunocytochemistry (ICC) (4), Immunoelectron Microscopy (IEM) (3)
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Conjugates
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Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), Biotin (6), PE,Cy5.5 (6), HRP (5), FITC (4), Alexa Flour 488 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), Gold (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy7 (3), RBITC (3)
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Epitopes
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beta chain (3), AA 57-87 (2), C-Term (2), Center (1)
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Alternatives 
