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Actin antibody
| Antigen | Actin |
| Synonyms |
A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G ...
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| Clonality | Monoclonal (1A4-5C5) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human, Sheep (Ovine), Cow (Bovine), Rabbit, Guinea Pig, Rat (Rattus), Frog, Snake, Carp |
| Application |
Alternatives Immunohistochemistry (IHC)
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| Catalog no. | ABIN125361 |
| Quantity | 500ul (100ug/ml approximately) (Variants) |
| Price | 198.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business days |
Additional Information
| Immunogen | BALB/C mice were injected with N-terminal decapeptide of alpha-smooth muscle actin for 1A4 and with purified rabbit striated muscle actin for 5C5. |
| Format | Ascites |
| Isotype | IgG2a Kappa & IgM (Matching secondary antibodies) |
| Clone | 1A4-5C5 |
| Specificity | This pan actin is useful in identifying tumors arising from muscle cells, i.e. leiomyosarcoma as well as rhabdomyosarcoma. It cross reacts with human, sheep, bovine, rabbit, guinea pig, rat, frog, snake and carp. |
| Synonyms | A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051 |
Application Details
| Application Notes | Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Skeletal muscle. Cellular localization: Cytoplasmic |
| Concentration | 100ug/ml approximately |
| Buffer | This antibody is supplied as diluted ascites containing sodium azide as preservative. |
| Storage | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Research Area | Extracellular Matrix |
| Restrictions | For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union |
Alternatives
Alternatives for antigen "Actin", type "Antibodies"
| Hosts | Mouse (58), Rabbit (18), Sheep (3) |
| Reactivities | Human (46), Chicken (23), Rat (Rattus) (19), Mouse (Murine) (12), Cow (Bovine) (10), Rabbit (9), Snake (7), Carp (5), Frog (5), Guinea Pig (5), Sheep (Ovine) (4), All Species (3), Dog (Canine) (3), Cat (Feline) (2), Pig (Porcine) (2), Xenopus laevis (2), Dictyostelium (1), Horse (Equine) (1), Physarum (1), Primate (1), Primates (1) |
| Applications | Western Blotting (WB) (54), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (38), Immunofluorescence (IF) (21), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), ELISA (16), Immunohistochemistry (IHC) (15), Immunoprecipitation (IP) (14), Immunocytochemistry (ICC) (7), Enzyme Immunoassay (EIA) (3), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (EM) (1), Radioimmunoassay (RIA) (1) |
| Conjugates | Biotin (6), Cy3 (1), FITC (1) |
| Epitopes | C-Term (6), pan (6), N-Term (3), Ab-55/53 (1), Asp244 (1), pTyr55/53 (1) |
