Placental Alkaline Phosphatase (PLAP) antibody

Antigen: Placental Alkaline Phosphatase (PLAP)

Synonyms: DOA1, PLAP, PLA2P, FLJ11281, FLJ12699, cb623, zgc:56568, zgc:66193, wu:fc45d08, wu:fi33e06, PLAA

Clonality: Monoclonal (8B6)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125362

Additional Information

Immunogen: Whole cells (Hep-2) expressing placental alkaline phosphatase.

Format: Ascites

Isotype: IgG2a kappa  (Matching secondary antibodies)

Clone: 8B6

Specificity: This antibody is specific to PLAP. This antibody stains germ cells and trophoblastic neoplasms, e.g. seminoma/dysgerminoma, embryonal carcinoma, yolk-sac tumor, choriocarcinoma and carcinoma in-situ of testis.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Positive Control: Placenta. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Enzymes

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union