CD79a Molecule, Immunoglobulin-Associated alpha (CD79A) antibody

Antigen: CD79a Molecule, Immunoglobulin-Associated alpha (CD79A)

Synonyms: IGA, MB-1, CD79A, IG-alpha

Clonality: Monoclonal (HM47-A9)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125365

Additional Information

Alternative name: CD79a

Immunogen: Synthetic polypeptide consisting of the amino acid sequence GTYQDVGSLNIADVQ.

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: HM47-A9

Specificity: This antibody is specific to human CD79a antigen complex, consisting of two polypeptides non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of polypeptide and immunoglobulins constitute the B cell antigen receptor. The two components of this complex are designated CD79a and CD79b. The CD79a antigen appears at the pre-B cell stage, early in maturation and persists until the plasma cell stage. The CD79a antigen is found in majority of acute leukemias of precursor B cell type, in B cell lines and B cell lymphomas.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union