Topoisomerase (DNA) II alpha 170kDa (TOP2A) antibody

Antigen: Topoisomerase (DNA) II alpha 170kDa (TOP2A)

Synonyms: TOP2, TP2A, TOP2A

Clonality: Monoclonal (SWT3D1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125366

Additional Information

Alternative name: Topoisomerase II-alpha

Immunogen: Synthetic polypeptide consisting of the amino acid sequence to amino acids 854-1447 of human topoisomerase II.

Format: Kappa Supern.

Isotype: IgG1  (Matching secondary antibodies)

Clone: SWT3D1

Specificity: This antibody is specific to alpha form of DNA topoisomerase II. It stains the nuclei of immunoreactive cells. This antibody is useful in the staining of small cell lung cancer, non-small cell lung carcinoma and breast carcinoma.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:15-1:35 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature antigen unmasking is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: DNA/RNA, Cell Cycle

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union