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Antibodies
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anti-Androgen Receptor (AR) antibody
Androgen Receptor (AR) antibody
| Antigen |
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| Synonyms |
KD, AIS, TFM, DHTR, SBMA, HYSP1, NR3C4, SMAX1, HUMARA, Tfm, AW320017, Andr, AR, DKFZp468G2216 |
| Clonality |
Monoclonal (AR 441) |
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Host
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Reactivity
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Human (83), Mouse (Murine) (40), Rat (Rattus) (36), Cow (Bovine) (19), Pig (Porcine) (19), Horse (Equine) (16), Rabbit (16), Chicken (15), Sheep (Ovine) (15), Dog (Canine) (7), Primate (2), Cat (Feline) (1), Chimpanzee (1), Goat (1), Lizard (1)
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Application
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Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (46), Immunofluorescence (IF) (34), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (29), Flow Cytometry (FACS) (18), ELISA (17), Immunohistochemistry (IHC) (14), Immunoprecipitation (IP) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Enzyme Immunoassay (EIA) (7), Dot Blot (Dot) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN125368 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
293.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Androgen Receptor
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Immunogen
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BALB/C mice were injected with a synthetic peptide, amino acid 299-315 (STEDTAEYSPFKGGYTK) of human androgen receptor.
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Format
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Supernatant
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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AR 441
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Specificity
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This antibody is specific to a protein of 110 kD, identified as androgen receptor. This antibody reacts with full length of AR and also with the newly described A form of the receptor. This antibody does not cross react with estrogen, progesterone or glucocorticoid receptors.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Prostate carcinoma. Cellular localization: Nuclear Other Applications: Western Blotting (1:50 to 1:100)
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Hormones, Receptors
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Androgen Receptor (AR)", type "Antibodies"
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Hosts
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Rabbit (71), Mouse (11), Goat (5), Rat (1)
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Reactivities
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Human (83), Mouse (Murine) (40), Rat (Rattus) (36), Cow (Bovine) (19), Pig (Porcine) (19), Horse (Equine) (16), Rabbit (16), Chicken (15), Sheep (Ovine) (15), Dog (Canine) (7), Primate (2), Cat (Feline) (1), Chimpanzee (1), Goat (1), Lizard (1)
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Applications
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Western Blotting (WB) (46), Immunofluorescence (IF) (34), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (29), Flow Cytometry (FACS) (18), ELISA (17), Immunohistochemistry (IHC) (14), Immunoprecipitation (IP) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Enzyme Immunoassay (EIA) (7), Dot Blot (Dot) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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N-Term (8), pSer213 (8), pSer650 (8), Ab-650 (5), Ab-1 (1), Ab-213 (1), Ab-363 (1), Ab-94 (1), pSer94 (1), pTyr363 (1)
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