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Antibodies
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anti-BCL2-Associated X Protein (BAX) antibody
BCL2-Associated X Protein (BAX) antibody
| Antigen |
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| Synonyms |
BCL2L4, bax, fj16e01, wu:fc50b10, wu:fj16e01, BAX, bax-A, xBax, xlbax, MGC76049, BAX-ALPHA |
| Clonality |
Monoclonal (2D2) |
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Host
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Reactivity
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Human (130), Mouse (Murine) (74), Rat (Rattus) (70), Dog (Canine) (32), Cow (Bovine) (31), Pig (Porcine) (31), Sheep (Ovine) (30), Chicken (1), Horse (Equine) (1), Monkey (1), Rabbit (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (89), Immunofluorescence (IF) (69), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (39), Immunoprecipitation (IP) (37), Flow Cytometry (FACS) (36), ELISA (18), Immunohistochemistry (IHC) (12), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2)
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| Catalog no. |
ABIN125369 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
311.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Bax
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Immunogen
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BALB/C mice were injected with a synthetic peptide amino acids 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.
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Format
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Supernatant
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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2D2
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Specificity
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This antibody recognizes a 24 kD protein identified as bax protein. This antibody is highly specific to bax and does not cross react with bcl-2 alpha or bcl-X protein. This antibody does not cross react with mouse and rat.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Histology Staining: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Hodgkin
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fractioncontaining sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Apoptosis/Necrosis
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "BCL2-Associated X Protein (BAX)", type "Antibodies"
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Hosts
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Mouse (77), Rabbit (68), Sheep (3)
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Reactivities
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Human (130), Mouse (Murine) (74), Rat (Rattus) (70), Dog (Canine) (32), Cow (Bovine) (31), Pig (Porcine) (31), Sheep (Ovine) (30), Chicken (1), Horse (Equine) (1), Monkey (1), Rabbit (1)
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Applications
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Western Blotting (WB) (89), Immunofluorescence (IF) (69), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (39), Immunoprecipitation (IP) (37), Flow Cytometry (FACS) (36), ELISA (18), Immunohistochemistry (IHC) (12), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2)
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Conjugates
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Biotin (8), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitopes
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N-Term (2), universal (1)
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