CD35 antibody

Antigen: CD35

Clonality: Monoclonal (E11)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125372

Additional Information

Immunogen: BALB/C mice were injected with intact human monocytes.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: E11

Specificity: This antibody is specific to a 210-220 kD protein, which is identified as the complement receptor 1 (CR1/CD35). This antibody recognizes as site in CR1 that is distal from the C3b- binding site, so that it is unable to block CR1 activity. This antibody does not cross react with CR2.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union