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Antibodies
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anti-Neural Cell Adhesion Molecule 1 (NCAM1) antibody
Neural Cell Adhesion Molecule 1 (NCAM1) antibody
| Antigen |
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| Synonyms |
CD56, NCAM, MSK39, ncam, fj56g09, wu:fj56g09, NCAM1, n-cam, LOC397761 |
| Clonality |
Monoclonal (1213C3-D5) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Flow Cytometry (FACS) (58), Western Blotting (WB) (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Immunoprecipitation (IP) (11), Immunocytochemistry (ICC) (7), Immunofluorescence (IF) (7), Enzyme Immunoassay (EIA) (5), ELISA (4), ELISA (Detection) (3), Immunohistochemistry (IHC) (2)
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| Catalog no. |
ABIN125376 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
206.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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CD56
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Immunogen
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BALB/C mice were injected with membrane preparation of a small cell lung carcinoma.
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Format
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Supernatant
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Isotype
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IgG1 kappa (Matching secondary antibodies)
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Clone
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1213C3-D5
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Specificity
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This antibody is specific to two proteins of 185 kD and 145 kD, identified as two isoforms of neural cell adhesion molecules (NCAM/CD56). NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Neuroblastoma. Cellular localization: Cell membrane
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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CD Antigens
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Neural Cell Adhesion Molecule 1 (NCAM1)", type "Antibodies"
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Hosts
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Mouse (76), Rabbit (5), Rat (1)
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Reactivities
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Human (75), Primates (11), Primate (10), Mouse (Murine) (3), Pig (Porcine) (3), Rat (Rattus) (3), Cow (Bovine) (1)
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Applications
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Flow Cytometry (FACS) (58), Western Blotting (WB) (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Immunoprecipitation (IP) (11), Immunocytochemistry (ICC) (7), Immunofluorescence (IF) (7), Enzyme Immunoassay (EIA) (5), ELISA (4), ELISA (Detection) (3), Immunohistochemistry (IHC) (2)
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Conjugates
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Biotin (7), FITC (7), PE (5), RPE (3), APC (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1), DY647 (1), PE,DY647 (1), Pacific Blue (1), PerCP (1), SPRD (1)
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