Cyclin D3 (CCND3) antibody
| Antigen |
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| Synonyms |
CYCE2, C78795, AA682053, AL024085, AW146355, 9230106B05Rik, MGC93643, MGC108760, zgc:103502, CCND3, MGC128700, LOC780426 |
| Clonality |
Monoclonal (DCS-28-1) |
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Host
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Reactivity
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Application
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Flow Cytometry (FACS) (21), Immunofluorescence (IF) (18), Western Blotting (WB) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunohistochemistry (IHC) (6), ELISA (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN125379 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
273.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Cyclin D3
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Immunogen
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Purified prokaryotic recombinant protein corresponding to full length cyclin D3.
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Format
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Supernatant
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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DCS-28-1
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Specificity
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This antibody is specific to a 34 kD protein identified as cyclin D3. It shows no cross reactivity with other cyclin Ds. Cyclin D3 can phophorylate pRB when associated with cdk4 and/or cdk6.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. This antibody is suitable for frozen tissue sections only and is not recommended for formalin fixed paraffin embedded tissue sections. Positive Control: Breast carcinoma. Cellular localization: Nuclear
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cell Cycle
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Cyclin D3 (CCND3)", type "Antibodies"
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Hosts
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Rabbit (25), Mouse (9)
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Reactivities
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Human (33), Rat (Rattus) (27), Mouse (Murine) (26), Dog (Canine) (19), Cow (Bovine) (18), Pig (Porcine) (18), Rabbit (17), Monkey (3), Horse (Equine) (2), Chicken (1)
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Applications
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Flow Cytometry (FACS) (21), Immunofluorescence (IF) (18), Western Blotting (WB) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunohistochemistry (IHC) (6), ELISA (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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Ab-283 (1), pThr283 (1)
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Alternatives 
