Granulocytes antibody

Antigen: Granulocytes

Clonality: Monoclonal (BM-2)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125382

Additional Information

Immunogen: BALB/c mice were immunized with nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: BM-2

Specificity: This antibody is specific to mature granulocytes. It does not react with other types of cells in human tissues. BM2 react with early precursor and mature forms of human myeloid cells. This antibody is useful in the detection of myeloid leukemia and granulocytic sarcoma.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Tonsil. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cell/Tissue Markers

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with Granulocyte antibody