Heat Shock Protein 70 (HSP70) antibody

Antigen: Heat Shock Protein 70 (HSP70)

Synonyms: HSPA6, HSP70B', HSP70, heat shock protein 70, CHLOROPLAST HEAT SHOCK PROTEIN 70-2, CPHSC70-2EAT SHOCK PROTEIN 70-2, HEAT SHOCK PROTEIN 70, HEAT SHOCK PROTEIN 70-7, HSC70-7, K9P8.5, K9P8_5, F19K16.12, F19K16_12, LOC100305036, hsc70

Clonality: Monoclonal (W27)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125383

Additional Information

Alternative name: Heat Shock Protein 70 (HSP-70)

Immunogen: BALB/C mice were injected with purified HSP70 protein.

Format: Supernatant

Isotype: IgG2a  (Matching secondary antibodies)

Clone: W27

Specificity: This antibody is specific to constitutive (HSP73) and inducible (HSP72) form of HSP70. HSP70 is found to be associated with steroid receptors, actin, p53, polyoma T antigen, nucleotides and other known proteins. HSP70 has been shown to be involved in protective role against thermal stress, cytotoxic drugs and other damaging conditions.

Synonyms: HSPA6, HSP70B', HSP70, heat shock protein 70, CHLOROPLAST HEAT SHOCK PROTEIN 70-2, CPHSC70-2EAT SHOCK PROTEIN 70-2, HEAT SHOCK PROTEIN 70, HEAT SHOCK PROTEIN 70-7, HSC70-7, K9P8.5, K9P8_5, F19K16.12, F19K16_12, LOC100305036, hsc70

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cytoplasmic and nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Metabolism, Heat Shock Proteins

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human breast carcinoma stained with Heat shock protein 70 antibody