Keratin High Molecular Weight antibody

Antigen: Keratin High Molecular Weight

Clonality: Monoclonal (DE-SQ)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125388

Additional Information

Alternative name: Cytokeratin (H.M.Weight)

Immunogen: BALB/C mice were injected with cytoskeletal fraction prepared from human gingiva by detergent/salt extraction.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: DE-SQ

Specificity: This antibody is specific to cytokeratin 13, 14, 15 and 16. This antibody stains squamous carcinomas and adenosquamous carcinomas but does not stain adenocarcinoma. This antibody is useful in differential staining of squamous carcinomas from adenocarcinomas and to differentiate benign and malignant tumors of prostate glands.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require digestion with Protease XXV at 1mg/ml PBS for 5 minutes at 370C. Positive Control: Skin. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human prostate stained with Cytokeratin H.M.W. antibody