Melan A (MLANA) antibody
| Antigen |
|
| Synonyms |
MART1, MART-1 |
| Clonality |
Monoclonal (A103) |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|
| Catalog no. |
ABIN125390 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
311.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
MART-1/Melan-A
|
|
Immunogen
|
BALB/C mice were injected with recombinant MART-1 protein.
|
|
Format
|
Supernatant
|
|
Isotype
|
IgG1 (Matching secondary antibodies)
|
|
Clone
|
A103
|
|
Specificity
|
This antibody is specific to a 20 kD protein known as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. This antibody does not cross react with MAGE-1 or tyrosinase protein. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes.
|
|
Application Notes
|
Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Melanoma. Cellular localization: Cytoplasmic Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)
|
|
Concentration
|
100ug/ml approximately
|
|
Buffer
|
This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
|
|
Storage
|
Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
|
|
Research Area
|
Cancer
|
|
Restrictions
|
For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
|
Alternatives for antigen "Melan A (MLANA)", type "Antibodies"
|
Hosts
|
Rabbit (16), Mouse (8)
|
|
Reactivities
|
Human (20), Horse (Equine) (1)
|
|
Applications
|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (21), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Western Blotting (WB) (7), Immunoprecipitation (IP) (3), Immunofluorescence (IF) (2), Immunohistochemistry (IHC) (2), ELISA (1), Enzyme Immunoassay (EIA) (1), Flow Cytometry (FACS) (1)
|
