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Myogenic Differentiation 1 (MYOD1) antibody

Antigen

Myogenic Differentiation 1 (MYOD1)

Synonyms PUM, MYF3, MYOD, bHLHc1, MyoD, MGC143434, pum, myf3, XMyoD, XtmyoD, MGC75960, MUF3, Myod-1, AI503393, myod, MGC136744, wu:fb57a01, zgc:136744, etID309723.25, MGC156574, MYOD1, myod1
Clonality Monoclonal (5-2F)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human

Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125391
Quantity 500ul  (100ug/ml approximately)  (Variants)
Price 232.00 $   Plus shipping costs $35.00
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Additional Information

Alternative name MyoD1
Immunogen BALB/C mice were injected with a recombinant mouse MyoD1 protein.
Format Supernatant
Isotype IgG2a  (Matching secondary antibodies)
Clone 5-2F
Specificity This antibody is specific to a 45 kD protein, which is identified as MyoD1. This antibody is specific to an epitope of amino acid 3-56 in the N-terminus of mouse MyoD1. This antibody does not react with myogenin, Myf5 or Myf6. MyoD1 stains the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is expressed strongly in the tumor cell nuclei of rhabdomyosarcomas.

Application Details

Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. This antibody is suitable for frozen tissues sections only. Positive Control: Tonsil. Cellular localization: Nuclear Other Applications: Immunoprecipitation (1:50)
Concentration 100ug/ml approximately
Buffer This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives

Alternatives for antigen "Myogenic Differentiation 1 (MYOD1)", type "Antibodies"
Hosts Rabbit (16), Mouse (12)
Reactivities Human (26), Mouse (Murine) (23), Rat (Rattus) (19), Chicken (6)
Applications Western Blotting (WB) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunoprecipitation (IP) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), ELISA (3), Enzyme Immunoassay (EIA) (3), Immunofluorescence (IF) (2), ELISA (Detection) (1)
Epitopes pSer200 (6)