»
Antibodies
»
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
| Antigen |
|
| Synonyms |
KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B |
| Clonality |
Monoclonal (DCS-72-F6) |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|
| Catalog no. |
ABIN125394 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
290.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
p27
|
|
Immunogen
|
BALB/C mice were injected with mouse recombinant p27 protein.
|
|
Format
|
Supernatant
|
|
Isotype
|
IgG1 (Matching secondary antibodies)
|
|
Clone
|
DCS-72-F6
|
|
Specificity
|
This antibody is specific to a protein of 27 kD known as p27Kip1 a cell cycle regulatory mitotic inhibitor. This antibody recognizes an epitope between amino acids 83-204 of p27. This antibody does not show any cross reactivity with other related mitotic inhibitors.
|
|
Application Notes
|
Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colan carcinoma. Cellular localization: Nuclear Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)
|
|
Concentration
|
100ug/ml approximately
|
|
Buffer
|
This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
|
|
Storage
|
Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
|
|
Research Area
|
Cancer, Cell Cycle
|
|
Restrictions
|
For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
|
Alternatives for antigen "Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)", type "Antibodies"
|
Hosts
|
Rabbit (36), Mouse (6), Goat (4)
|
|
Reactivities
|
Human (42), Mouse (Murine) (22), Rat (Rattus) (20), Dog (Canine) (3), Cat (Feline) (1), Monkey (1)
|
|
Applications
|
Western Blotting (WB) (35), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (21), Immunoprecipitation (IP) (10), ELISA (9), Immunohistochemistry (IHC) (9), Enzyme Immunoassay (EIA) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Dot Blot (Dot) (1), Immunofluorescence (IF) (1)
|
|
Epitopes
|
pThr187 (4), internal (2), C-Term (1), T198 (1), pS10 (1), pS178 (1), pSer178 (1), pT157 (1), pT187 (1), pThr157 (1), pThr198 (1)
|
