Thyroid and Lung Epithelial Marker (NKX2-1) antibody

Antigen: Thyroid and Lung Epithelial Marker (NKX2-1)

Clonality: Monoclonal (8G7G3-1)

Host: Mouse

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125397

Additional Information

Alternative name: Thyroid and Lung Epithelial Marker (TF-1)

Immunogen: BALB/C mice were injected with a recombinant rat protein.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: 8G7G3-1

Specificity: This antibody is specific to a 40 kD protein, which is identified as Thyroid transcription factor (TTF-1). TTF-1 is expressed in epithelial cells of the thyroid gland and lung. TTF- 1 stains primary lung adenocarcinoma and small cell carcinoma but does not stain colon and breast carcinoma.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Lung. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cell/Tissue Markers

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human lung stained with TTF-1 antibody