P57 antibody

Antigen: P57

Clonality: Monoclonal (KP10)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125403

Additional Information

Immunogen: BALB/C mice were injected with mouse recombinant p57Kip2 protein.

Format: Supernatant

Isotype: IgG2b kappa  (Matching secondary antibodies)

Clone: KP10

Specificity: This antibody is specific to a protein of 57 kD known as p57Kip2 a cell cycle regulatory mitotic inhibitor. This antibody does not cross react with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes and is negative regulator of cell proliferation.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colan carcinoma. Cellular localization: Nuclear Other Applications: Immunoprecipitation (1:50)

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer, Cell Cycle

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human colon carcinoma stained with p57Kip2 antibody