CD63 antibody
| Antigen |
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| Clonality |
Monoclonal (NK1-C3) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN125410 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
271.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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Smooth plasma membrane fraction of MeWo cells.
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Format
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Ig-fraction
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Isotype
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IgG1 kappa (Matching secondary antibodies)
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Clone
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NK1-C3
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Specificity
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This antibody is specific to 53 kD protein, expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also expressed on granulocytes, B cells and T cells.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:100 to 1:200 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Melanoma. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied purified antibody containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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CD Antigens
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "CD63", type "Antibodies"
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Hosts
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Mouse (78), Rabbit (3), Rat (1)
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Reactivities
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Human (74), Cow (Bovine) (3), Mouse (Murine) (1)
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Applications
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Flow Cytometry (FACS) (58), Immunoprecipitation (IP) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Western Blotting (WB) (18), Immunofluorescence (IF) (12), Immunocytochemistry (ICC) (11), Immunohistochemistry (IHC) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Radioimmunoassay (RIA) (1)
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Conjugates
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FITC (11), APC (6), PE (5), Biotin (2), PE,DY647 (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1), PE-Dyomics 647 (1), Pacific Blue (1), PerCP (1), RPE (1)
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