Prolactin-Induced Protein (PIP) antibody

Antigen: Prolactin-Induced Protein (PIP)

Clonality: Monoclonal (23A3)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125414

Additional Information

Alternative name: Gross Cystic Disease Fluid Protein-15 (GCDFP15)

Immunogen: Recombinant protein encoding the excreted domain of human GCDFP-15.

Format: Supernatant

Isotype: IgG2a kappa  (Matching secondary antibodies)

Clone: 23A3

Specificity: This antibody is specific to 15 kD monomer protein called Gross Cystic Disease Fluid Protein-15 (GCDFP-15). Gross cystic disease of the breast is benign premenopausal disorder in which cysts are predominant. These cysts appear to be formed from excessive apocrine cystic secretions, one of which is GCDFP-15. This antibody can be used in for the staining of breast carcinoma, salivary duct carcinoma and apocrine epithelia.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10 to 1:20 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human skin stained with GCDFP15 antibody