Vascular Endothelial Cell Growth Factor (VEGF) antibody

Antigen: Vascular Endothelial Cell Growth Factor (VEGF)

Clonality: Monoclonal (VG1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125415

Additional Information

Immunogen: BALB/C mice were injected with a recombinant VEGF protein.

Format: Ascites

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: VG1

Specificity: This antibody is specific to a cluster of proteins between 19-22kD (reduced). VEGF is a homodimeric disulfide-linked glycoprotein involved in angiogenesis, which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to the immunostaining. Positive Control: Astrocytoma. Cellular localization: Cytoplasmic, cell membrane, extracellular matrix

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified from ascites fluid by protein G chromatography, containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human angiosarcoma stained with VEGF antibody