Heat Shock Protein 60 (HSP60) antibody

Antigen: Heat Shock Protein 60 (HSP60)

Synonyms: HLD4, CPN60, GROEL, HSP60, HSP65, SPG13, HuCHA60, 60kDa, Hsp60, 12, BP5, Cpn60, Dmhsp60, G62, Hsp60A, Mmp-P1, l(1)10Ac, l(1)BP5, l(1)G8, l(1)HM21, l(1)L12, l(1)dp025, DmelCG12101, CG12101, HEAT SHOCK PROTEIN 60, HEAT SHOCK PROTEIN 60-3B, HSP60-3B, 23.m06276

Clonality: Monoclonal (LK1)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Hamster, Chicken

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN125420

Additional Information

Alternative name: Heat Shock Protein 60 (HSP 60)

Immunogen: BALB/C mice were injected with recombinant HSP 60 protein.

Format: Ig-fraction

Isotype: IgG1  (Matching secondary antibodies)

Clone: LK1

Specificity: This antibody is specific to 60 kD protein, HSP60. HPS 60 is a potential antigen in a number of autoimmune diseases including human arthritis. This antibody recognizes only mammalian HSP60 and not bacterial HSP60. It cross reacts with human, mouse, rat, hamster and chicken.

Synonyms: HLD4, CPN60, GROEL, HSP60, HSP65, SPG13, HuCHA60, 60kDa, Hsp60, 12, BP5, Cpn60, Dmhsp60, G62, Hsp60A, Mmp-P1, l(1)10Ac, l(1)BP5, l(1)G8, l(1)HM21, l(1)L12, l(1)dp025, DmelCG12101, CG12101, HEAT SHOCK PROTEIN 60, HEAT SHOCK PROTEIN 60-3B, HSP60-3B, 23.m06276

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cytoplasmic Other Applications: Immunofluorescence, Immunoprecipitation, Western Blotting.

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Metabolism, Heat Shock Proteins

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human breast carcinoma stained with Heat shock protein 60 antibody