Thrombospondin (TSP) antibody
| Antigen |
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| Synonyms |
CT31613, D-TSP, DTSP, SP295, tsp, DmelCG11326, CG11326, GB13833 |
| Clonality |
Monoclonal (A6-1) |
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Host
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Reactivity
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 Alternatives Human, Cow (Bovine), Pig (Porcine), Horse (Equine), Dog (Canine), Sheep (Ovine), Mouse (Murine), Rat (Rattus)
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Application
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Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN125422 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
313.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
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Format
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Ig-fraction
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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A6-1
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Specificity
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This antibody is specific to 450 kD protein, a non-reduced and 170 to 180 kD, reduced form of Thrombospondin. Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells. This antibody cross reacts with human, cow, pig, horse, dog, sheep, mouse and rat.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Secretory granules, Golgi complex, endoplasmic reticulum Other Applications: Immunofluorescence, Immunoprecipitation, Western Blotting.
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Extracellular Matrix
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Thrombospondin (TSP)", type "Antibodies"
