TIMP Metallopeptidase Inhibitor 2 (TIMP2) antibody

Antigen: TIMP Metallopeptidase Inhibitor 2 (TIMP2)

Synonyms: CSC-21K, MGC84288, MGC108274, TIMP-2, TIMP2, timp2

Clonality: Monoclonal (3A4)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Rabbit, Cow (Bovine), Guinea Pig

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125425

Additional Information

Alternative name: Tissue Inhibitor of Metalloproteinase-2 (TIMP-2)

Immunogen: BALB/C mice were injected with a synthetic peptide from the N-terminal region of human TIMP-2.

Format: Ig-fraction

Isotype: IgG2a kappa  (Matching secondary antibodies)

Clone: 3A4

Specificity: This antibody is specific to 21 kD protein known as TIMP-2. TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family. TIMPs are of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72 kD Type IV collagenase (MMP-2) and active form of 92 kD Type IV collagenase (MMP-9). TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. This antibody cross reacts with mouse, rat, rabbit, cow and Guinea pig.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma. Cellular localization: Cytoplasmic Other Applications: Western Blotting

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer, Extracellular Matrix, Inflammation

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human pancreatic tumor stained with TIMP-2 antibody