Polymerase (DNA Directed), beta (POLB) antibody

Antigen: Polymerase (DNA Directed), beta (POLB)

Synonyms: polB, MGC130858, polb, MGC69287, POLB, MGC174347, MGC194077, wu:fa97a05, zgc:109924, MGC128623, MGC125976, A430088C08Rik

Clonality: Monoclonal (61)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN125426

Additional Information

Alternative name: Deoxyribonucleic Acid (DNA) Polymerase beta

Immunogen: BALB/C mice were injected with DNA polymerase beta protein.

Format: Ig-fraction

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 61

Specificity: This antibody is specific to 39 kD protein known as DNA polymerase beta. DNA polymerase beta comprises an amino-terminal 8 kD domain and a carboxy-terminal 31 kD domain. DNA Polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Testis. Cellular localization: Nuclear Other Applications: Western Blotting

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: DNA/RNA, Enzymes, Metabolism

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human testis stained with DNA polymerase beta antibody