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Antibodies
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anti-X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1...
X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1) antibody
| Antigen |
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| Synonyms |
RCC, Xrcc-1, MGC102556, xrcc1, MGC80909, XRCC1 |
| Clonality |
Monoclonal (02-05-1933) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (47), Immunoprecipitation (IP) (29), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (28), Immunofluorescence (IF) (27), Flow Cytometry (FACS) (20), ELISA (6), Immunohistochemistry (IHC) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), ELISA (Detection) (3), Immunocytochemistry (ICC) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN125431 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
342.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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X-Ray Repair Cross Complementing (XRCC1)
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Immunogen
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BALB/C mice were injected with recombinant human XRCC1 protein.
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Format
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Ig-fraction
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Isotype
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IgG2b (Matching secondary antibodies)
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Clone
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02-05-1933
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Specificity
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This antibody is specific to 85kD kD protein known as XRCC1. X-ray Repair Cross Complementing (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNS ligase III through the C-terminal 96 amino acids and to DNA polymerase beta through the N-terminal half. In testis XRCC1 is expressed at high levels. This antibody cross reacts with rat.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Testis. Cellular localization: Nuclear Other Applications: Western Blotting, Immunoprecipitation, Immunofluorescence
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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DNA/RNA
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1)", type "Antibodies"
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Hosts
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Rabbit (34), Mouse (33)
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Reactivities
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Human (67), Rat (Rattus) (26), Mouse (Murine) (22), Horse (Equine) (3), Rabbit (3), Cow (Bovine) (2), Dog (Canine) (2), Pig (Porcine) (2)
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Applications
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Western Blotting (WB) (47), Immunoprecipitation (IP) (29), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (28), Immunofluorescence (IF) (27), Flow Cytometry (FACS) (20), ELISA (6), Immunohistochemistry (IHC) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), ELISA (Detection) (3), Immunocytochemistry (ICC) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (4), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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