Xeroderma Pigmentosum, Complementation Group A (XPA) antibody

Antigen: Xeroderma Pigmentosum, Complementation Group A (XPA)

Synonyms: XP1, XPAC, Xpac, AI573865

Clonality: Monoclonal (12F5)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125436

Additional Information

Alternative name: Xeroderma Pigmentosum Group A Protein (XPA)

Immunogen: BALB/C mice were injected with recombinant human XPA protein.

Format: Ig-fraction

Isotype: IgG2a kappa

Clone: 12F5

Specificity: This antibody reacts with a 40 kD protein known as XPA (Xeroderma pigmentosum group A) protein. XPA specifically recognizes the UV or chemically damaged DNA lesions and triggers the nucleotide excision repair process. XPA binds to the replication protein A (RPA) or excision repair cross complementing 1 protein (ERCC1).

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear Other Applications: Western Blotting

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union