MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) antibody

Antigen: MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2)

Synonyms: FCC1, COCA1, HNPCC, LCFS2, HNPCC1, AI788990

Clonality: Monoclonal (25D12)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125437

Additional Information

Alternative name: Mismatch Repair Protein (MSH2)

Immunogen: BALB/C mice were injected with recombinant human MSH2 protein.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 25D12

Specificity: This antibody reacts with a 102 kD protein known as MSH2 (Mismatch Repair protein 2). MSH2 protein is involved in the initial recognition of mismatched nucleotides during the post replication mismatch repair process. Loss of MSH2 function leads to the accumulation of replication errors, which in turn may be responsible for the multiple mutations required for multistage carcinogenesis.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union