Transcription Factor AP-2 alpha (Activating Enhancer Binding Protein 2 Alpha) (TFAP2A) antibody

Antigen: Transcription Factor AP-2 alpha (Activating Enhancer Binding Protein 2 Alpha) (TFAP2A)

Synonyms: ap2, AP-2, xap2, XAP-2, ap2tf, tfap2, ap-2alpha, tfap2alpha, BOFS, AP2TF, TFAP2, FLJ51761, AP-2alpha, cb93, fb83f04, fc31a07, wu:fb83f04, wu:fc31a07, tfap2a-a, TFAP2A, tcfap2a, MGC151772, Ap-2, ap2a

Clonality: Monoclonal (A6-2-2)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN125438

Additional Information

Alternative name: AP-2 Transcription Factor

Immunogen: BALB/C mice were injected with a peptide from the C-terminus of AP-2 alpha.

Format: Ig-fraction

Isotype: IgG1  (Matching secondary antibodies)

Clone: A6-2-2

Specificity: This antibody reacts with a 48 kD protein. This antibody reacts with both alpha and beta forms of AP-2 transcription factor. AP-2 genes are expressed in many human breast cancer cell lines. AP-2 binding sites are present in both c-erbB-2 and estrogen receptor promoters.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:5-1:10 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1 mM EDTA buffer, pH 8.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union