CD22 (CD22) antibody
| Antigen |
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| Synonyms |
SIGLEC2, FLJ22814, SIGLEC-2, MGC130020 |
| Clonality |
Monoclonal (DCS-120-DCS-121) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Western Blotting (WB) (55), Immunoprecipitation (IP) (32), Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Enzyme Immunoassay (EIA) (7), ELISA (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunohistochemistry (IHC) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN125442 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
314.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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cdc25A
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Immunogen
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BALB/C mice were injected with purified recombinant CDC25A.
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Format
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Ig-fraction
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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DCS-120-DCS-121
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Specificity
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This antibody reacts with a 65 kD protein known as CDC25A. The activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S phase and G2 for CDC25B. CDC25C is mainly expressed in G2 phase. This antibody cross reacts with rat.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear membrane Other Applications: Immunoprecipitation, Western Blotting
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cell Cycle, Cancer, Kinases/Phosphatases
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "CD22 (CD22)", type "Antibodies"
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Hosts
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Rabbit (55), Mouse (25)
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Reactivities
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Human (74), Rat (Rattus) (37), Mouse (Murine) (34), Dog (Canine) (16), Chicken (15), Cow (Bovine) (15), Rabbit (15)
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Applications
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Western Blotting (WB) (55), Immunoprecipitation (IP) (32), Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Enzyme Immunoassay (EIA) (7), ELISA (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunohistochemistry (IHC) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (3)
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