Minichromosome Maintenance Complex Component 7 (MCM7) antibody

Antigen: Minichromosome Maintenance Complex Component 7 (MCM7)

Synonyms: MCM2, CDC47, HsMcm7, P85MCM, P1CDC47, PNAS-146, CDABP0042, P1.1-MCM3, MGC93853, MCM7

Clonality: Monoclonal (47DC141)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Xenopus laevis

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN125445

Additional Information

Alternative name: cdc47 (MCM7)

Immunogen: BALB/C mice were injected with recombinant human CDC47 protein.

Format: Ig-fraction

Isotype: IgG1  (Matching secondary antibodies)

Clone: 47DC141

Specificity: This antibody reacts with a 80 kD protein known as hCDC47. hCDC47 is a member of MCM family, which allows DNA to replicate once per cell cycle. Expression of hCDC47 protein has been found in the nuclei of proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contain more nuclear hCDC47-positive cells than their normal counterparts. This antibody cross reacts with mouse, rat, dog and Xenopus.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear Other Applications: Immunoprecipitation, Western Blotting

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cell Cycle

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with CDC47 antibody