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Antibodies
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anti-Cyclin-Dependent Kinase 5 (CDK5) antibody
Cyclin-Dependent Kinase 5 (CDK5) antibody
| Antigen |
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| Synonyms |
PSSALRE, Crk6, AW048668, CDK5, DmCdk5, DmelCG8203, CG8203, MGC140720 |
| Clonality |
Monoclonal (DC17-DC34) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Western Blotting (WB) (58), Immunofluorescence (IF) (36), Immunoprecipitation (IP) (29), Flow Cytometry (FACS) (21), ELISA (14), Enzyme Immunoassay (EIA) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), ELISA (Detection) (4), Immunohistochemistry (IHC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN125447 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
314.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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cdk5
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Immunogen
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BALB/C mice were injected with a recombinant cdk5 protein.
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Format
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Ig-fraction
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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DC17-DC34
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Specificity
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This antibody reacts with a 31 kD protein known as cdk5. cdk-5 is expressed in both cycling and noncycling cells and is present in numerous mammalian tissues. Neuronal and muscle cells contain the highest amount of this protein. Cdk5 activity is involved in terminal differentiation of neurons and muscle cells. This antibody cross reacts with mouse and rat.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Brain. Cellular localization: Cytoplasmic, nuclear Other Applications: Immunoprecipitation, Western Blotting
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cell Cycle
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Cyclin-Dependent Kinase 5 (CDK5)", type "Antibodies"
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Hosts
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Rabbit (44), Mouse (32)
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Reactivities
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Human (70), Mouse (Murine) (34), Rat (Rattus) (33), Cow (Bovine) (18), Pig (Porcine) (17), Dog (Canine) (3), Horse (Equine) (1), Monkey (1), Rabbit (1)
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Applications
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Western Blotting (WB) (58), Immunofluorescence (IF) (36), Immunoprecipitation (IP) (29), Flow Cytometry (FACS) (21), ELISA (14), Enzyme Immunoassay (EIA) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), ELISA (Detection) (4), Immunohistochemistry (IHC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (4), C-Term P201 (1)
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