Neuregulin 1 (NRG1) antibody

Antigen: Neuregulin 1 (NRG1)

Synonyms: GGF, HGL, HRG, NDF, ARIA, GGF2, HRG1, HRGA, SMDF, HRG4, DKFZp779N0541, DKFZp779N1944, Hgl, GGFII, HRGalpha, 6030402G23Rik, D230005F13Rik, NRG1, MGC154949, nrg1-A, nrg1, NRG-1, wu:fm70e01, DKFZp459A1838

Clonality: Monoclonal (7D5)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN125448

Additional Information

Alternative name: Heregulin/NDF/GGF/Neuregulin

Immunogen: BALB/C mice were injected with a recombinant extracellular domain of rat NDF protein.

Format: Ig-fraction

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 7D5

Specificity: This antibody reacts with a 44 kD protein known as Heregulin or neu differentiation factor (NDF). Heregulin binds to e-erbB-3 and c-erbB-4 receptors on mammary and neuronal cells with low and high affinities, respectively. HRG/NDF exists in several isoforms, which are classified in two groups, alpha and beta that differ in their EGF- like domain and in receptor binding affinity. This antibody cross reacts with mouse and rat.

Synonyms: GGF, HGL, HRG, NDF, ARIA, GGF2, HRG1, HRGA, SMDF, HRG4, DKFZp779N0541, DKFZp779N1944, Hgl, GGFII, HRGalpha, 6030402G23Rik, D230005F13Rik, NRG1, MGC154949, nrg1-A, nrg1, NRG-1, wu:fm70e01, DKFZp459A1838

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Prostate carcinoma. Cellular localization: Cytoplasmic, intercellular spaces Other Applications: Immunoprecipitation, Western Blotting (1:100-500).

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Growth Factors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human prostate carcinoma stained with Heregulin antibody