Calponin antibody

Antigen: Calponin

Clonality: Monoclonal (CALP)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125450

Additional Information

Immunogen: BALB/C mice were injected with crude human uterus extract.

Format: Ig-fraction

Isotype: IgG1  (Matching secondary antibodies)

Clone: CALP

Specificity: This antibody reacts with a 34 kD protein known as Calponin. Calponin, a calmodulin, binds tropomyosin and F-actin and is thought to be involved in the regulation of smooth muscle contraction. Expression of Calponin is restricted to smooth muscle cells. Two isoforms of Calponin exist whose molecular weights are 34 KD and 29 kD. 29 kD isoform is primarily restricted to the muscle of the urogenital tract. This antibody cross reacts with rat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require digestion of tissue sections with trypsin and followed by high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human breast stained with Calponin antibody