Thyroid Hormone Receptor, Alpha1/Alpha2 (THRA1/2) antibody

Antigen: Thyroid Hormone Receptor, Alpha1/Alpha2 (THRA1/2)

Synonyms: LOC397942, thra1, thra-A, nr1a1-A, THRB, NR1A2

Clonality: Monoclonal (1718)

Host: Mouse

Reactivity: Human, Rat (Rattus), Mouse (Murine), Pig (Porcine)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN125454

Additional Information

Alternative name: Thyroid Hormone Receptor (alpha1/alpha2)

Immunogen: BALB/C mice were injected with synthetic peptide corresponding to aa 13-32 from N-terminus of human TRalpha1.

Format: Ig-fraction

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 1718

Specificity: This antibody reacts with a 50 KD (TRalpha1) and 60 kD (TRalpha1) proteins. It does not react with beta isotype. Thyroid hormone receptors (TR) are ligand dependent members of the steroid/retinoic acid superfamily of transcription factors. The two genes encoding TRs identified to date, TRalpha and TRbeta, have been mapped to human chromosomes 17 and 3, respectively. This antibody cross reacts with mouse, rat and pig.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma. Cellular localization: Nuclear Other Applications: Western Blotting

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Hormones, Receptors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union